Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods

Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods

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Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis.The non-extraction method COSMETICS is fast as roots are directly depolymerized using acidic transmethylation.However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g.

, membranes) present in roots.For the solvent-extraction method, roots are first delipidated before transmethylation.This method is longer but allows separation of soluble and polymerized root lipids.This protocol is optimized for tissue culture- or soil-grown Arabidopsis thaliana plants, but can be Chino Trousers used with roots of other plants.